Lani Misalucha was born into a musical family. Her father Benjamin Bayot had been an operatic tenor, her mother Esperanza Dimalanta was a coloratura soprano, sister May is a band vocalist, sister Karlyn is an actress (Munting Paraiso) who also plays violin and flute, while brothers Novi and Osi play guitar and piano. Tutored in classical vocals by her own parents, Misalucha and her sisters expanded their musical horizons to various genres and styles, and developed dynamic versatility
Misalucha acquired her basic education from Rafael Palma Elementary School and PCU Union High School. At age 16, Misalucha won the top prize in a Gospel music competition. At age 20, she sang with the Andres Bonifacio Choir of the Cultural Center of the Philippines. She eventually joined various bands as a vocalist or back-up singer, beginning with the group "Prelude" and later the group "Law of Gravity" whose bandleader Bodjie Dasig gave Misalucha her first single, entitled "More than I Should" (1993).She also recorded "Sakayan ng Jeep," Bodjie Dasig's swoony ballad in R&B style. Misalucha would later do the lounge circuits as a soloist and lend her voice to numerous commercial jingles and karaoke tapes, thus earning her the epithet, "Multiplex Queen."
After Misalucha obtained her degree in Business Management from the Philippine Christian University, music took a backseat in the early 1990s as she prioritized her role as wife to Noli Misalucha (an IT expert, former PLDT employee and Mormon pastor) and mother to two kids, Lian and Louven.
Misalucha is a member of The Church of Jesus Christ of Latter-Day Saints, and actively supports church-related humanitarian programs in the Philippines and in the United States. She and singer Martin Nievera held charity concerts for the benefit of Hurricane Katrina victims in New Orleans